Procedure
Part A:
Parts of the Light Microscope

• Answer questions in the 1-4 in the lab Report.
• Use the terms listed here to label the figure of the microscope below.  Fill these answers in for Part A of your Lab Report.

Image of a compound light microscope with arrows pointing to parts A-L. The labeled parts are: A. the lens the viewer looks through to see the specimen, B. a rotating turret that houses the objective lenses, C. cylindrical lenses which can be rotated that range in power from 4X to 100X, D. The flat platform where the slide is placed, F. lever that controls the amount of light reaching the specimen, G. gathers and focuses light from the illuminator onto the specimen being viewed, H. the light source for a microscope, I. connects the eyepiece to the objective lenses, J. connects the body tube to the base of the microscope, K. brings the specimen into general focus, L. fine-tunes the focus and increases the detail of the specimen.

• Arm
• Condenser
• Coarse focus knob
• Fine focus knob
• Head
• Iris diaphragm
• Iris diaphragm lever
• Lamp
• Objective lenses
• Ocular lenses
• Rotating nosepiece
• Stage

Part B:
Using the Light Microscope

• Use the information in the "Using the Light Microscope" section of the Lab Manuel, the slides of the printed letter "e" below and Figure 1.3 to answer the questions in Part B of the Lab Report.
• Use the black arrow to move to the next image.

This is  the orientation of the letter "e" when it is placed on the microscope stage. Notice what happens to the orientation (direction) of this image when viewed through the microscope on the next slides.

the figure shows the unturned orientation of the lowercase English alphabet letter “e” when placed on a compound microscope stage.
an inverted magnified image of letter e viewed under 4X objective lens
magnified inverted part of letter e viewed under 10X objective lens
magnified part of letter e under 40X objective lens occupies the viewing field

Part C:
Total Magnification & Numerical Aperture (NA)

•Use the definition of total magnification and numerical aperture given in the Lab 1 reading and Figure 1.3 below to answers question 23 and complete Table 1.1 in Part C of the Lab Report.
•The arrow is showing the working distance for one of the lenses.

the figure shows four objective lenses 4X, 10X, 40X, and 100X. An example of working distance is shown by an arrow between the slide and 4X lens. The image shows less space between the bottom of the lens and the slide as the magnification increases.

Part D:
Resolution & Contrast

• Answer the Questions in Part D of the Lab Report.

Some of the protists you may see in pond water mentioned in Fig 2.3 include the bi-lobed green algae called algae cosmarium, the slipper-shaped motile ciliated paramecium, the motile photosynthetic discoidal unicellular organism called euglena, the filamentous green alga called spirogyra, the spherical colonial green alga called volvox, the horn-shaped or trumpet-like ciliate, organism called stentor, the pennate diatom called nitzchia, the filamentous segmented green alga called penium, the oval/bean-shaped green alga called navicula, the bell-shaped ciliates organism with stalks called vorticella, and the star-shaped green algae called staurastrum.


• The lenses on the microscope have been adjusted for best contrast.
• Use Figure 1.4 to help you identify the organisms in pond water video.
NOTE - not all the organisms shown in Figure 1.4 are in the video.
• Then select one of the organisms you identified and observe it in more detail.
• Answer questions in Part D of the Lab Report.

Watch Pond water Video

Part E:
Whole Mounts & Serial Sections

• Answer the Questions in Part E of the Lab Report.

After you have submitted your Lab Report Via Google please go to Blackboard, select the Lab for this week and the "Grading Rubric" . Then select "Write Submission" and type in "I have submitted my Lab Report Via Google docs"
This will allow me to enter a grade that you can see on Blackboard in your grade book for each lab.